Product details

Analytical sensitivity: 1ng/mL

Test range: 1,029-750ng/mL

Sample type/volume

• Plasma: 2 µL
• Serum: 2 µL
• Floating: 100μL

Hands-on time: 1 hour 20 minutes

Time to result: 4 hours 45 minutes

Homogeneous (without washing): Do not

Inter-assay CV: <12%

Intra-assay CV: <10%

Instrument: Colorimetric microplate reader

Product size: 96 tests

Content

• Pre-coated 96-well plate
• Standard
• Assay Diluent Concentrate
• Biotinylated detection antibody
• SAV-HRP
• wash buffer
• chromogen
• stop solution
• Adhesive plate covers

Send conditions: Wet or dry ice

Storage: 2-8°C

About this team

The Human Transferrin ELISA Kit quantifies Hu transferrin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both native and recombinant Hu transferrin.

Principle of the method

The Human Transferrin Solid Phase Sandwich ELISA (Enzyme-Linked Immunosorbent Assay) is designed to measure the amount of bound target between a pair of matched antibodies. A target-specific antibody has been pre-coated on the wells of the supplied microplate.

Samples, standards, or controls are then added to these wells and bound to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added which reacts with the enzyme-antibody-target complex to produce a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original sample.

Destination information

Transferrins (Tf) are an approximately 80 kDa blood plasma glycoprotein synthesized by the liver. They are iron-binding carrier proteins that can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from the sites of heme absorption and degradation to those of storage and utilization.

Transferrin is the main iron transport protein in the blood and, under normal conditions; approximately one-third of total blood transferrin contains bound iron. Measurement of blood transferrin levels can be used as an indicator of the iron-carrying capacity of the blood and of abnormalities of iron metabolism, such as anaemia, iron overload, and hemochromatosis. Serum transferrin may also have an additional role in stimulating cell proliferation.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested against criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See the manual for more information on validation.

For research use only. It should not be used in diagnostic procedures. Not for resale without express permission.